Food emulsions stabilized by proteins and emulsifiers: A review of the mechanistic explorations.

The stability of food emulsions is the basis for other properties. During their production and processing, emulsions tend to become unstable due to their thermodynamic instability, and it is usually necessary to add emulsifiers and proteins to stabilize emulsions. It becomes crucial to study the intrinsic mechanisms of emulsifiers and proteins and their joint stabilization of food emulsions. This paper summarizes the research on intrinsic mechanisms of food emulsions stabilized by emulsifiers and proteins in recent years. The destabilization and stabilization of emulsions are related to the added surfactants. The properties, type, and concentration of emulsifiers determine the stability of emulsions, and the emulsifiers can be classified into different types (e.g., ionic or nonionic, solid or liquid) according to their properties and sources. The physicochemical properties of proteins (e.g., spatial conformation, hydrophobicity) and the composition of proteins can also determine the stability of emulsions, and emulsions stabilized by emulsifiers and proteins together not only depend on these factors but also have a great relationship with the mutual combination and competition between the two. The instability and stability of emulsions are related to factors such as interfacial interaction forces, the rheological nature of the interface, and the added surfactant.

Real-world effectiveness and predictors of super-responders to dupilumab in a Chinese uncontrolled asthma cohort.

Background: Dupilumab has been shown to be effective in clinical trials for moderate-to-severe uncontrolled asthma. However, the efficacy of dupilumab in the real world and the prediction of treatment response have not been well studied in patients with asthma. Objective: To investigate the efficacy of dupilumab and explore predictors of super-responders in a Chinese retrospective cohort. Methods: From January 2021 through December 2022, the patients with uncontrolled asthma who were treated with dupilumab for 4 months were included. Symptom control, type 2 inflammatory biomarkers, and lung function were collected at baseline and follow-up for efficacy assessment. Super-responders were defined as exacerbation-free, off maintenance of oral corticosteroids (mOCS), and with a score of the five-item Asthma Control Questionnaire (ACQ-5) of <0.5. The uni- and multivariable logistic regressions were used to construct predictive models for super-responders based on baseline features. Results: A total of 53 patients were included. After 4 months treatment, the median (interquartile range [IQR]) ACQ-5 score decreased from 1.8 (1.6-2.4) to 0.4 (0.2-0.8) (p < 0.001), the median (IQR) number of exacerbations, from 0.0 (0.0-1.0) to 0.0 (0.0-0.0) (p = 0.005). The median (IQR) dose of mOCS (prednisone equivalent) decreased from 15.0 mg/day (8.8-22.5 mg/day) to 2.5 mg/day (0.0-10.0 mg/day) (p = 0.008) in nine patients who were receiving mOCS. All efficacy assessment parameters, including sputum eosinophil were significantly improved, while blood eosinophil count did not decline (530 cells/mm³ [300-815 cells/mm³] versus 560 cells/mm³ [220-938 cells/mm³], p = 0.710). After taking dupilumab, 25 of 53 patients (47.2%) achieved a super-response. The age of onset < 42 years (odds ratio [OR] 7.471 [95% confidence interval {CI}, 1.286-43.394) and the baseline fractional exhaled nitric oxide (FeNO) of 25-50 ppb (OR 35.038 [95% CI, 3.104-395.553]) predicted super-responders, which showed a C-index of 0.822 (95% CI, 0.697-0.947). Conclusion: Dupilumab significantly improved symptom control, type 2 inflammatory markers, and lung function in Chinese patients with uncontrolled asthma. Airway eosinophils, rather than blood eosinophils, can be a reliable indicator of therapeutic efficacy. The early-onset asthma as well as the medium-high level of baseline FeNO contributed to the prediction of super-responders.

Investigation of Optoelectronic Functional Crystals: Crystal Growth, Properties and Applications.

Optoelectronic functional crystals facilitate the conversion of light and electricity, inspiring breakthroughs in the fabrication of optoelectronic devices [...].

Gabapentin alleviated the cough hypersensitivity and neurogenic inflammation in a guinea pig model with repeated intra-esophageal acid perfusion.

OBJECTIVE: The anti-tussive effect of gabapentin and its underlying neuromodulatory mechanism were investigated via a modified guinea pig model of gastroesophageal reflux-related cough (GERC). METHODS: Intra-esophageal perfusion with hydrochloric acid (HCl) was performed every other day 12 times to establish the GERC model. High-dose gabapentin (48 mg/kg), low-dose gabapentin (8 mg/kg), or saline was orally administered for 2 weeks after modeling. Cough sensitivity, airway inflammation, lung and esophagus histology, levels of substance P (SP), and neurokinin-1 (NK1)-receptors were monitored. RESULTS: Repeated intra-esophageal acid perfusion aggravated the cough sensitivity in guinea pigs in a time-dependent manner. The number of cough events was significantly increased after 12 times HCl perfusion, and the hypersensitivity period was maintained for 2 weeks. The SP levels in BALF, trachea, lung, distal esophagus, and vagal ganglia were increased in guinea pigs receiving HCl perfusion. The intensity of cough hypersensitivity in the GERC model was significantly correlated with increased SP expression in the airways. Both high and low doses of gabapentin administration could reduce cough hypersensitivity exposed to HCl perfusion, attenuate airway inflammatory damage, and inhibit neurogenic inflammation by reducing SP expression from the airway and vagal ganglia. CONCLUSIONS: Gabapentin can desensitize the cough sensitivity in the GERC model of guinea pig. The anti-tussive effect is associated with the alleviated peripheral neurogenic inflammation as reflected in the decreased level of SP.

The glycopatterns of Pseudomonas aeruginosa as a potential biomarker for its carbapenem resistance.

IMPORTANCE: Bacterial surface glycans are an attractive therapeutic target in response to antibiotics; however, current knowledge of the corresponding mechanisms is rather limited. Antimicrobial susceptibility testing, genome sequencing, and MALDI-TOF MS, commonly used in recent years to analyze bacterial resistance, are unable to rapidly and efficiently establish associations between glycans and resistance. The discovery of new antimicrobial strategies still requires the introduction of promising analytical methods. In this study, we applied lectin microarray technology and a machine-learning model to screen for important glycan structures associated with carbapenem-resistant P. aeruginosa. This work highlights that specific glycopatterns can be important biomarkers associated with bacterial antibiotic resistance, which promises to provide a rapid entry point for exploring new resistance mechanisms in pathogens.

Transmission Electron Microscopy Peeled Surface Defect of Perovskite Quantum Dots to Improve Crystal Structure.

Transmission electron microscopy (TEM) is an excellent characterization method to analyze the size, morphology, crystalline state, and microstructure of perovskite quantum dots (PeQDs). Nevertheless, the electron beam of TEM as an illumination source provides high energy, which causes morphological variation (fusion and melting) and recession of the crystalline structure in low radiolysis tolerance specimens. Hence, a novel and facile strategy is proposed: electron beam peel [PbBr6]4- octahedron defects from the surface of QDs to optimize the crystal structure. TEM and high-angle annular dark-field scanning TEM (HAADF) tests indicate that the [PbBr6]4- octahedron would be peeled from the surface of QDs when QDs samples were irradiated under high-power irradiation, and then a clear image would be obtained. To avoid interference from a protective film of "carbon deposits" on the surface of the sample when using high resolution TEM, amorphous carbon film (15-20 nm) was deposited on the surface of QDs film and then characterized by TEM and HAADF. The detection consequences showed that the defection of PbBr2 on the surface of QDs will gradually disappear with the extension of radiation time, which further verifies the conjecture.

Efficient Surfactant-Mediated Photovoltaic Manipulation of fL-Scale Aqueous Microdroplets for Diverse Optofluidic Applications on LiNbO3 Platform.

The electrodeless biocompatible manipulation of femtoliter-scale aqueous microdroplets remains challenging. The appropriate isolation of electrostatic charges from femtoliter-scale aqueous microdroplets is crucial for electrodeless optoelectronic manipulation based on space-charge-density modulation. Here, surfactant-mediated photovoltaic manipulation is proposed, where the surfactant layers self-assembled at the water-oil and oil-Lithium niobate interfaces are employed to isolate photovoltaic charges. The reduced electrostatic attenuation, remarkable hydrophobicity, and strong electrical breakdown suppression of the surfactant layers enable the stable and swift manipulation of femtoliter-scale aqueous microdroplets using µW-level laser in oil media. By virtue of the surfactant-mediated photovoltaic manipulation, a controllable merging/touching/detaching switch of aqueous microdroplets by adjusting the laser illumination intensity and position is realized and the cascading biochemical operations and microreactions of aqueous microdroplets and microdroplet strings are demonstrated. To demonstrate its potential in photonic Micro-Electro-Mechanical-System assemblies, the end coupling of a focused-laser-beam into a ZnO microrod leveraging the refraction effect occurring at the water/oil interface is demonstrated. Moreover, because of the selective permeability of the droplet-interface-bilayer developed between the touching microdroplets, in situ adjustment of the size of the microdroplets and the fluorescent solute contained in the microdroplets are achieved, aiming at constructing multicomponent fluorescent microdroplets with tunable whispering-gallery-mode characteristics.

Metformin regulates bone marrow stromal cells to accelerate bone healing in diabetic mice.

Diabetes mellitus is a group of chronic diseases characterized by high blood glucose levels. Diabetic patients have a higher risk of sustaining osteoporotic fractures than non-diabetic people. The fracture healing is usually impaired in diabetics, and our understanding of the detrimental effects of hyperglycemia on fracture healing is still inadequate. Metformin is the first-line medicine for type 2 diabetes (T2D). However, its effects on bone in T2D patients remain to be studied. To assess the impacts of metformin on fracture healing, we compared the healing process of closed-wound fixed fracture, non-fixed radial fracture, and femoral drill-hole injury models in the T2D mice with and without metformin treatment. Our results demonstrated that metformin rescued the delayed bone healing and remolding in the T2D mice in all injury models. In vitro analysis indicated that compromised proliferation, osteogenesis, chondrogenesis of the bone marrow stromal cells (BMSCs) derived from the T2D mice were rescued by metformin treatment when compared to WT controls. Furthermore, metformin could effectively rescue the impaired detrimental lineage commitment of BMSCs isolated from the T2D mice in vivo as assessed by subcutaneous ossicle formation of the BMSC implants in recipient T2D mice. Moreover, the Safranin O staining of cartilage formation in the endochondral ossification under hyperglycemic condition significantly increased at day 14 post-fracture in the T2D mice receiving metformin treatment. The chondrocyte transcript factors SOX9 and PGC1α, important to maintain chondrocyte homeostasis, were both significantly upregulated in callus tissue isolated at the fracture site of metformin-treated MKR mice on day 12 post-fracture. Metformin also rescued the chondrocyte disc formation of BMSCs isolated from the T2D mice. Taken together, our study demonstrated that metformin facilitated bone healing, more specifically bone formation and chondrogenesis in T2D mouse models.

Dielectrophoresis-electrophoresis transition during the photovoltaic manipulation of water microdroplets on LiNbO3:Fe platform.

The abrupt behaviors of microdroplets during the LN-based photovoltaic manipulation may cause the transient instability and even failure of the microfluidic manipulation. In this paper, we perform a systematical analysis on the responses of water microdroplets to laser illumination on both naked and PTFE-coated LN:Fe surface, and find that the abrupt repulsive behaviors of the microdroplets are due to the electrostatic transition from the dielectrophoresis (DEP) to electrophoresis (EP) mechanism. Charging of the water microdroplets through the Rayleigh jetting from electrified water/oil interface is suggested as the cause of the DEP-EP transition. Fitting the kinetic data of the microdroplets to the models describing the motion of the microdroplets under the photovoltaic field yields the charging amount depending on the substrate configuration (∼1.7 × 10-11 and 3.9 × 10-12 C on the naked and PTFE-coated LN:Fe substrates), and also reveals the dominance of the EP mechanism in the co-existence of the DEP and EP mechanisms. The outcome of this paper will be quite important to the practicalization of the photovoltaic manipulation in LN-based optofluidic chips.

Excitation-Dependent Tunable White Light of ns2 Ions Doped Rb2SnCl6 Vacancy Ordered Double Perovskite.

Single-matrix white light-emitting diodes are still a challenge. Achieving tunable white light emission from lead-free perovskites attracts much attention. Herein, nanoscale Rb2SnCl6 (RSC) vacancy ordered double perovskite were synthesized by an optimized precipitation method. Further, using ns2 ions (Bi3+, Te4+, and Sb3+) doped RSC vacancy ordered double perovskite to obtain broadband blue, yellow-green, and orange-red emission. The color temperature adjustable high-quality white light emission based on the codoping strategy is obtained by controlling the doping ratio of Bi3+ and Te4+. Additionally, the white light-emitting diodes encapsulated by this single matrix white luminescent material exhibit excellent stability. Combined with theoretical calculations, it is elucidated that these high-efficiency emissions are not only derived from the unique electronic transition of ns2 ions but also related to the energy band properties and the crystal field. This work shows that ns2 ions doped RSC vacancy ordered double perovskite can meet the needs of different luminous colors and color temperature tunable white light emission. Meanwhile, it is a strong competitor to replace the lead-based perovskite.

Efficient and stable perovskite-silicon tandem solar cells through contact displacement by MgFx.

The performance of perovskite solar cells with inverted polarity (p-i-n) is still limited by recombination at their electron extraction interface, which also lowers the power conversion efficiency (PCE) of p-i-n perovskite-silicon tandem solar cells. A MgFx interlayer with thickness of ~1 nanometer at the perovskite/C60 interface favorably adjusts the surface energy of the perovskite layer through thermal evaporation, which facilitates efficient electron extraction and displaces C60 from the perovskite surface to mitigate nonradiative recombination. These effects enable a champion open-circuit voltage of 1.92 volts, an improved fill factor of 80.7%, and an independently certified stabilized PCE of 29.3% for a monolithic perovskite-silicon tandem solar cell ~1 square centimeter in area. The tandem retained ~95% of its initial performance after damp-heat testing (85°C at 85% relative humidity) for >1000 hours.

Efficient Broadband Yellow-Green Emission of Vacancy Halide Double Perovskites Through the Ion-Exchanged Strategy.

Broadband high-efficiency luminescent materials have become a hot spot in lead-free perovskite research. There are relatively few broadband yellow-green phosphors with both ultraviolet and blue excitation ranges, which make them more suitable for phosphor-converted white LEDs. Through the ion-exchanged strategy, Cs2Hf1-xTexCl6 (CH1-xTxC) vacancy halide double perovskites were successfully prepared at room temperature. Using different excitation ranges of CH1-xTxC, two types of high-quality white LEDs are obtained. By combining density functional theory calculations and experiments, it is proved that this bright broadband yellow-green emission (photoluminescence quantum yield of 83.46%) is not only derived from the ion transitions of Te4+ but also exhibits the inherent characteristics of self-trapped exciton emission. Our results not only broaden the application fields of lead-free halide perovskites but also provide further insights into the luminescence mechanism.

3D Photovoltaic Router of Water Microdroplets Aiming at Free-Space Microfluidic Transportation.

So far, microfluidic navigation based on space-charge modulation is limited in a two-dimensional (2D) substrate plane. In this paper, a three-dimensional (3D) photovoltaic water-microdroplet router based on a superhydrophobic LiNbO3:Fe crystal is reported. This router employs the repulsive electrostatic force induced by the positive photovoltaic charges generated under focused laser illumination and permits traveling microdroplets to be routed in both in-plane and out-of-plane ways. By analyzing the dynamic process of microdroplet routing, it is found that the microdroplets can gain positive charges through traveling on a superhydrophobic surface and that the positive photovoltaic charges exert an electrophoretic (EP) force on the microdroplets being charged and make them either routed inside the 2D substrate plane or jump out of the 2D plane through electrostatic ballistic ejection. The laser-illumination and microdroplet-size dependence of the deflecting parameters of the in-plane microdroplet routing as well as the jumping trajectory of the out-of-plane routing are investigated. An electrostatic kinetic model is established for both routing ways, and the simulation based on this model predicts well the experimental dependence. A few examples of cascaded free-space microfluidic transportation using the 3D photovoltaic router are demonstrated, showing the potential of this technique in future biological applications.

Hydrophobic-substrate based water-microdroplet manipulation through the long-range photovoltaic interaction from a distant LiNbO3:Fe crystal.

Development of photovoltaic water-microdroplet manipulation using LN:Fe crystals has to meet the requirement of the hybrid and heating-avoided design of biological lab-on-chips. To fulfill this, we demonstrate a successful manipulation of a water microdroplet on a hydrophobic substrate by utilizing the long-range photovoltaic interaction from a distant LN:Fe crystal (see Visualization 1). The maximal manipulation distance (MMD) is found to be dependent on the laser-illumination intensity at the LN:Fe crystal and it can be tuned up to a sub-centimeter level (∼4 mm). Basing on the two-center model of light-induced charge transport in the LN:Fe crystal, we establish an analytic model to describe the force balance during the microdroplet manipulation under a long-range photovoltaic interaction. Either shortening the manipulation distance or increasing the illumination intensity can enhance the photovoltaic interaction and increase the velocity of the microdroplet being manipulated. An abrupt shape change followed by a fast repelling movement of the water microdroplet is observed under a strong photovoltaic interaction (see Visualization 2).

Internal Cylinder Identification Based on Different Transmission of Longitudinal and Shear Ultrasonic Waves.

We have built a Fizeau fiber interferometer to investigate the internal cylindrical defects in an aluminum plate based on laser ultrasonic techniques. The ultrasound is excited in the plate by a Q-switched Nd:YAG laser. When the ultrasonic waves interact with the internal defects, the transmitted amplitudes of longitudinal and shear waves are different. The experimental results show that the difference in transmission amplitudes can be attributed to the high frequency damping of internal cylinders. When the scanning point is close to the internal defect, the longitudinal waves attenuate significantly in the whole defect area, and their amplitude is always smaller than that of shear waves. By comparing the transmitted amplitudes of longitudinal and shear waves at different scanning points, we can achieve a C scan image of the sample to realize the visual inspection of internal defects. Our system exhibits outstanding performance in detecting internal cylinders, which could be used not only in evaluating structure cracks but also in exploring ultrasonic transmission characteristics.

Identification of Differentially Methylated Regions Associated with a Knockout of SUV39H1 in Prostate Cancer Cells.

Epigenetic alterations, such as histone methylations, affect the pathogenesis of tumors including prostate cancer (PCa). Previously, we reported that metformin reduced SUV39H1, a histone methyltransferase of H3 Lys9, to inhibit the migration of PCa cells. Since histone methylation is functionally linked to DNA methylation, we speculate that the knockout of the SUV39H1 gene will affect the genomic DNA methylation profile to regulate PCa cell migration and invasion. The genome-wide DNA methylation level is lower in SUV39H1 knockout (KO) cells than wild-type (WT) ones. However, the methylation levels in functional regions of CpG Islands (CGI), 5' untranslated region (UTR5), and exon regions are higher in KO cells than WT cells. Analysis of differentially methylated regions (DMRs) identified 1241 DMR genes that have differential methylation on CG sites when comparing the KO and WT samples. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes Pathways analysis showed that knockout of SUV39H1 affects gene sets and pathways that are heavily involved in cell shapes, cell recognition, adhesion, motility, and migration. Our study suggests that SUV39H1 plays an important role in PCa migration via the epigenetic regulation of methylation on CG sites, and is a novel and legitimate target to inhibit PCa cell migration.

Locomotor activity patterns of takin (Budorcas taxicolor) in a temperate mountain region.

Understanding locomotor activity patterns would reveal key information about an animal's foraging strategy, energy budget and evolutionary adaptation. We studied the locomotor activity patterns of the takin (Budorcas taxicolor) in a temperate mountain region in China using GPS radio-collar technology from 1 July 2014 to 30 June 2015. Our research showed that takin had a bimodal crepuscular locomotor activity pattern, with an especially obvious movement peak at dusk. The takins showed significant seasonal differences in their movement rates, with the lowest movement rate in winter. The animals also showed sexual differences in their movement rates. In spring, the female movement rate was significantly higher than that of males during daytime, while during nighttime the movement rate of males was higher than that of females. The male movement rate was significantly higher than that of females in summer. The movement rate of the takins were correlated to microenvironment temperature and normalized difference vegetation index (NDVI) in each season. These findings suggest that takin could adjust locomotor activity levels adapt to reproductive requirements, temperature variation and forage variability.

Photovoltaic splitting of water microdroplets on a y-cut LiNbO3:Fe crystal coated with oil-infused hydrophobic insulating layers.

We demonstrate the successful photovoltaic splitting of water microdroplets on a $y$y-cut ${{\rm LiNbO}_3}:{\rm Fe}$LiNbO3:Fe substrate coated with an oil-infused hydrophobic layer. The temporal evolution of the microdroplet contact angle upon a central illumination and the distinct behaviors of two sub-droplets during a following boundary illumination reveal that both electrowetting and electroosmotic effects induced by the dipolar photovoltaic potential on the substrate contribute to the water microdroplet splitting. The reciprocal relationship between the splitting time and the illumination intensity verifies the inherent photovoltaic nature of the water microdroplet splitting. The splitting time is found to be linearly dependent on the initial microdroplet size. These points are quite important to the practicalization of lithium niobate (LN)-based microfluidic chips in the biological field.

Retraction Note: Interactions between Th1 cells and Tregs affect regulation of hepatic fibrosis in biliary atresia through the IFN-γ/STAT1 pathway.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Visible-light-assisted condensation of ultrasonically atomized water vapor on LiNbO3:Fe crystals.

Optically massive trapping of the moisture in the air into an adjacent surface is a potential technique in the fields of bacterial adhesion and microfluidic generation, which is quite important to the development of LN-based biological lab-on-chips. Here we demonstrate on a LiNbO3:Fe substrate the visible-light-assisted condensation of the water vapor in a flowing stream created by an ultrasonic atomizer. Through analyzing the dynamic processes of the visible-light-assisted water condensation at different illumination intensities, it is found that the extent of the water condensation, the bending angle of water vapor trails and the interaction range of the condensation effect are highly dependent on the illumination intensity. According to these findings and the simulated trajectories of the water vapor stream at different illumination intensities, we propose that this visible-light-assisted water condensation is an aggregation process of tiny water droplets driven by the dielectrophoretic interaction of inhomogeneous photovoltaic field and also an electrostatic screening course of photovoltaic charges through the charged evaporation of condensed water. The prolonged condensation of water vapor after a high-intensity illumination and that of oil vapor at a super-low evaporation rate are also studied, and the agreement between the simulation and experimental results reinforces the above mechanism. The reported technique, employing the inexpensive, safe-for-cell visible laser beam, is quite convenient for the controllable generation of various biological microdroplets, and thus it is promising for the microfluidic functionality integration of LN-based biological lab-on-chips.